About the application of centrifuge in medical laboratory

The hospital's laboratory will use a centrifuge. Many patients go to the hospital and the doctor will draw blood, then use the vacuum tube to take it and take it to the laboratory for testing.

Attentive people will find that most doctors will put the vacuum blood collection tube into the centrifuge for centrifugation. The following is the process of using the centrifuge in the hospital laboratory.

First, after the doctor takes the blood collection tube, it will be placed on the vacuum blood collection tube shelf. When a certain amount is reached, it will be placed in a centrifuge for centrifugation.

The number of centrifugations must be even, because one tube can't be centrifuged. The centrifuge needs to be balanced during the centrifugation process. Although some centrifuges are automatically balanced, it is impossible to say that a tube can be centrifuged, but it is relatively The difference is not a large weight to maintain normal operating conditions.

Centrifuges have a very high force during centrifugation, which can lead to accidents if unbalanced.

Therefore, when the doctor puts the test tube into the centrifuge, it is symmetrical. After the centrifuge is started, the speed of the rotation is slowly increased, and then the set speed is reached. When the set time is reached, the centrifugal machine automatically stops. Due to the inertia, the speed of the centrifuge will slowly drop from the set speed and will not stop immediately to zero.

After the centrifuge is stopped, the door cover cannot be opened immediately. It needs to wait until the speed of the centrifuge will be zero. The medical centrifuge will highlight the current speed on the screen.

When the speed is zero, the door will open automatically. At this time, the doctor will take out the centrifuged blood collection tube, put it on the test tube rack, and then send it to check.

Centrifugal time and relative centrifugal force (RCF): Clinical chemical analysis When the blood specimen is centrifuged, RCF (1000 ~ 1200) × g, centrifugation time is 5 ~ 10min.

Temperature Control Centrifugation: Heat generation during centrifugation is not conducive to analyte stability. Clinical chemical analysis Blood samples must be centrifuged with a temperature-controlled centrifuge. Some temperature-dependent analytes (such as adrenocorticotropic hormone, cyclic adenosine monophosphate, catecholamines, etc.) should be separated at 4 ° C;

For analytes without special temperature requirements, the centrifugation temperature should be set at 20 °C ~ 22 °C; the temperature below 15 °C can artificially increase the blood potassium measurement; the refrigerated specimen must be centrifuged at the required temperature.

About re-centrifugation: The specimen is centrifuged once. If it needs to be centrifuged again, it should be shortly separated from the last centrifugation; for blood samples containing separated substances, it can never be centrifuged again.

Biochemistry: Centrifugation at 3000 rpm for 7 minutes or 2400 rpm for 15 minutes.

Coagulation: Centrifugation at 3500 rpm for 10 minutes

Immunization: Centrifuge at 3500 rpm for 5 minutes