The Application of Centrifuges in Cancer Cell Culture

Release time:

2014-06-30

Overview:

First, during cultivation, strict adherence to aseptic techniques is essential. All instruments, culture media, and reagents must be sterilized and should not be exposed to air for extended periods. Operations must be performed on a clean bench to prevent contamination. It is strictly forbidden to touch the interior of sterilized containers with your hands or blow into them with your mouth.

To begin with, the isolated cancer-fighting cells are placed into a culture dish and then cultured in a specialized environment—such as an infrared CO2 incubator (water-jacket or direct-heating type)—to promote their growth. This incubator offers precise control over CO2 concentration and temperature, maintains high humidity, and provides thorough sterilization, thereby creating an optimal culture environment for the cells. During the 24-hour culture period, we closely observe the growth and proliferation of the cancer-fighting cells. Since the growth patterns vary among different individuals, it is common practice to use a centrifuge to separate and isolate the samples, selecting the most promising cancer-fighting cells for further cultivation.

　Next, the cells are cultured in a CO2 incubator for approximately one week. During this period, results are typically observed at intervals of 24 to 48 hours. Once the culture is proceeding smoothly, the culture dishes are removed from the CO2 incubator and transferred onto a laminar flow hood, where they are pipetted into centrifuge tubes. The tubes are then placed in the horizontal rotor of a centrifuge for a final round of centrifugation, thereby yielding the anti-cancer cells required by cancer patients.

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