Special Design of High-Speed Refrigerated Centrifuge for Cell Separation
Release time:
2017-02-16
Overview:
High-speed refrigerated centrifuge
High-speed refrigerated centrifuge It is a subcategory within the broader centrifuge family, primarily used for separating cells and other biological materials. Therefore, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd. has specially designed these centrifuges to meet the specific requirements of the substances being separated. For instance, due to the limited length of centrifuge tubes, each type of cell can only travel a short effective distance under the influence of the centrifugal force field, making it impossible to completely separate all cells in a single step. In the initial stages of separation, it is advisable to employ methods with lower resolution but capable of recovering all cells. If high-resolution methods were used from the outset, components of interest might be discarded along with impurities that have similar physicochemical properties. During the separation process, the number of steps should be minimized as much as possible, while carefully preserving the integrity and viability of the cells to prevent loss of function. Additionally, contamination must be minimized, and attention should be paid to ensuring the effectiveness of the centrifugation method.
A high-speed refrigerated centrifuge is a specialized instrument that uses centrifugal force to separate and precipitate mixed liquids (containing solid particles). Commonly used laboratory centrifuges include low-speed, high-speed, and low- and high-speed refrigerated centrifuges, as well as various models of ultrahigh-speed analytical and preparative refrigerated centrifuges. A high-speed refrigerated centrifuge is a high-speed centrifuge equipped with a refrigeration system. The temperature inside the centrifuge chamber of such a machine can typically be lowered below zero degrees Celsius. With rotational speeds reaching over 10,000 rpm, in addition to possessing the performance and structural features of a conventional refrigerated centrifuge, High-speed refrigerated centrifuge The angular rotors used are typically made of titanium alloy or aluminum alloy. The centrifuge tubes are rigid polyethylene plastic products with caps. These types of centrifuges are commonly used to collect microorganisms, cell debris, cells, large organelles, sulfate precipitates, and immunoprecipitates, among other materials. So, what are the key issues that need to be given special attention when operating a high-speed refrigerated centrifuge?
The key issues that need to be focused on when operating a high-speed refrigerated centrifuge include:
1. The volume of solution loaded into the centrifuge tubes must be appropriate: for stainless steel tubes without caps, fill only up to 2/3; for plastic tubes, fill up to the shoulder. The tube caps must be tightly sealed—leakage is absolutely not permitted. Empty tubes may deform during centrifugation. When using organic solvents with plastic tubes, ensure compliance with applicable regulations.
2. Centrifuge tubes must be placed in pairs and strictly balanced, with a deviation of less than 0.1 g.
3. Do not allow the rotor to spin idly without a sample. Always use the handle when placing or removing the rotor to prevent it from slipping and falling. Place the rotor gently and ensure it is securely seated. When unscrewing the handle, hold the handle with your hand and rotate only the rotor itself. The rotor cover must be tightly sealed; centrifugation is prohibited without a properly closed cover.
4. High-speed refrigerated centrifuge Do not open the lid during centrifugation, and do not lean against or hold onto the centrifuge. If you hear any unusual noises or notice vibrations, immediately stop the machine.
5. After using the rotor, it must be promptly removed from the rotor chamber, dried thoroughly, and carefully cleaned inside the tube holes using a hole-cleaning rod. If any solution has spilled, it must be thoroughly cleaned up. Wipe away any condensation inside the rotor chamber, open the door to allow the chamber to air-dry.
6. Use of the centrifuge requires a reservation and must be logged after use.
7. Those who have not undergone training and assessment are not permitted to use it.
8. Select the appropriate rotor and rotational speed; never operate at speeds exceeding the specified limits.
9. Select an appropriate temperature—typically 4℃—and, except for organic solvents, do not go below zero to avoid freezing, which could damage the centrifuge tubes and rotor.
10. Before using the rotor, you must clean the tube holes thoroughly with a hole-cleaning rod and carefully inspect them for cracks or white spots at the bottom of the holes. If any are found, discard the rotor immediately.
The above is about High-speed refrigerated centrifuge Some basic information—this knowledge is essential for customers who are looking to purchase or learn more about this type of centrifuge. We hope it will be helpful to everyone. It’s recommended that you familiarize yourself with the basics before contacting suppliers, so you can communicate more effectively and avoid taking the wrong path!
A high-speed refrigerated centrifuge is a specialized instrument that uses centrifugal force to separate and precipitate mixed liquids (containing solid particles). Commonly used laboratory centrifuges include low-speed, high-speed, and low- and high-speed refrigerated centrifuges, as well as various models of ultrahigh-speed analytical and preparative refrigerated centrifuges. A high-speed refrigerated centrifuge is a high-speed centrifuge equipped with a refrigeration system. The temperature inside the centrifuge chamber of such a machine can typically be lowered below zero degrees Celsius. With rotational speeds reaching over 10,000 rpm, in addition to possessing the performance and structural features of a conventional refrigerated centrifuge, High-speed refrigerated centrifuge The angular rotors used are typically made of titanium alloy or aluminum alloy. The centrifuge tubes are rigid polyethylene plastic products with caps. These types of centrifuges are commonly used to collect microorganisms, cell debris, cells, large organelles, sulfate precipitates, and immunoprecipitates, among other materials. So, what are the key issues that need to be given special attention when operating a high-speed refrigerated centrifuge?
The key issues that need to be focused on when operating a high-speed refrigerated centrifuge include:
1. The volume of solution loaded into the centrifuge tubes must be appropriate: for stainless steel tubes without caps, fill only up to 2/3; for plastic tubes, fill up to the shoulder. The tube caps must be tightly sealed—leakage is absolutely not permitted. Empty tubes may deform during centrifugation. When using organic solvents with plastic tubes, ensure compliance with applicable regulations.
2. Centrifuge tubes must be placed in pairs and strictly balanced, with a deviation of less than 0.1 g.
3. Do not allow the rotor to spin idly without a sample. Always use the handle when placing or removing the rotor to prevent it from slipping and falling. Place the rotor gently and ensure it is securely seated. When unscrewing the handle, hold the handle with your hand and rotate only the rotor itself. The rotor cover must be tightly sealed; centrifugation is prohibited without a properly closed cover.
4. High-speed refrigerated centrifuge Do not open the lid during centrifugation, and do not lean against or hold onto the centrifuge. If you hear any unusual noises or notice vibrations, immediately stop the machine.
5. After using the rotor, it must be promptly removed from the rotor chamber, dried thoroughly, and carefully cleaned inside the tube holes using a hole-cleaning rod. If any solution has spilled, it must be thoroughly cleaned up. Wipe away any condensation inside the rotor chamber, open the door to allow the chamber to air-dry.
6. Use of the centrifuge requires a reservation and must be logged after use.
7. Those who have not undergone training and assessment are not permitted to use it.
8. Select the appropriate rotor and rotational speed; never operate at speeds exceeding the specified limits.
9. Select an appropriate temperature—typically 4℃—and, except for organic solvents, do not go below zero to avoid freezing, which could damage the centrifuge tubes and rotor.
10. Before using the rotor, you must clean the tube holes thoroughly with a hole-cleaning rod and carefully inspect them for cracks or white spots at the bottom of the holes. If any are found, discard the rotor immediately.
The above is about High-speed refrigerated centrifuge Some basic information—this knowledge is essential for customers who are looking to purchase or learn more about this type of centrifuge. We hope it will be helpful to everyone. It’s recommended that you familiarize yourself with the basics before contacting suppliers, so you can communicate more effectively and avoid taking the wrong path!
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