The function of the centrifuge is to achieve a certain centrifugal force through a certain rotation speed, and then separate the substance, which can be solid liquid or liquid liquid, so as long as the centrifuge equipment has no defects, the operation is normal during operation, and the cover is correct, basically not It will cause pollution during the separation test.
Therefore, centrifuges are widely used in various laboratory research institutes throughout the country and even the world, as well as hospitals, beauty salons and so on.
The following is a brief explanation of the centrifuge separation process:
The experimental sample is centrifuged at very high speeds, and the components in the mixture are affected by the centrifugal force, so that the higher density particles move away from the axis, while the smaller density particles move toward the axis.
These particles are deposited at the bottom of the tube to form a so-called precipitate, so that the separated sample, or the remainder of the supernatant, can be used for further processing or analysis. The centrifuge simulates the linear acceleration generated by the motion of the object through the centripetal acceleration principle. By precisely controlling the speed of the centrifuge and the distance from the sensitive center of mass to the center of rotation, a stable centripetal acceleration output is generated at a constant speed. The comparison of the sensor output value with the setpoint of the centrifuge.
Of course, compared to understanding the separation principle of the centrifuge, in fact, as a centrifuge user, it is better to focus on the use of the centrifuge.
Consider the temperature before starting to centrifuge your sample. If you are using a refrigerated centrifuge, you need to let the internal temperature of the instrument reach your desired value before starting, or use another method to cool the rotor.
Before the centrifugation is about to begin, make sure that all of your caps are tightly closed and will not loosen.
When placing the tube into the rotor, make sure that the weight of each sample and the other relative position are the same.
If you only have one centrifuge tube, find another equal weight centrifuge tube to ensure balance.
If you have three centrifuge tubes of the same weight, you can place them in a triangular position to ensure a balance.
Weight balance is very important in centrifugation. The rotor of the centrifuge has a high dynamic energy during high-speed motion. If not properly balanced, the entire centrifuge unit will be out of its position, causing serious damage.
Once you have determined that the rotor and cover are fixed, start centrifugation and do not leave until the speed reaches the maximum speed you set. If you find a problem, find an experienced experimenter to help you.
After centrifugation, you should see your biological sample in the sediment at the bottom of the centrifuge tube. It has been separated from the remaining liquid called the supernatant.
The above is about the explanation of the centrifugation principle of the centrifuge and the precautions for the operation of the centrifuge. Although the centrifuge is now getting smaller and smaller, the rotation speed is getting higher and higher, so it is more cautious in the use process, the safety test is The essential.
