The laboratory centrifuge is a commonly used scientific instrument for separating and purifying suspended substances of different physical density and different physical scales in liquid samples by using the relative centrifugal force generated by the high-speed rotation of the rotor. Because the rotor is in continuous high-speed operation when the centrifuge is working, if it does not operate according to the prescribed process, it may cause a safety risk. Laboratory centrifuge safety risk: The main drive motor shaft is deformed or broken. The hazard and preventive measures of this risk are as follows.
Main drive motor shaft deformation or broken shaft
Whether the sample loading balance is closely related to the operation safety of the centrifuge, unbalanced loading will cause abnormal vibration and noise, causing damage to the motor bearing system. In severe cases, the motor shaft may be deformed or even a shaft breakage may occur. Suddenly broken shaft, the centrifugal rotor loses support, and the chaotic collision in the centrifugal chamber may cause the centrifuge to flip and shift. If there are personnel or equipment next to it, it will cause serious accidents such as casualties and equipment damage.
When using various centrifuges, all centrifuge tubes, centrifuge caps, hanging baskets and adapters must be precisely balanced on the balance beforehand. The weight deviation during balancing must not exceed the range specified in the centrifuge instructions. The precision balance requirement does not exceed 0.1g, and the conventional application requires no more than 1g. Hanging baskets and adapters are usually marked with weight. Be careful not to confuse the matching hanging baskets and accessories during the balancing process. The balance tube used for load balancing must be filled with materials of similar density, for example, by centrifuging bacteria from the medium, which can be equilibrated with water. However, it is not possible to balance cesium chloride with water. Try to avoid loading a single number of centrifuge tubes in the rotor. When the rotor is only partially loaded, it should be evenly distributed so that the load is evenly distributed on the rotor body. When loading the solution, it should be carried out according to the specific operation instructions of various centrifuges. According to the nature and volume of the solution to be centrifuged, select the appropriate centrifuge tube. When using the uncovered centrifuge tube, the liquid should not be loaded too much, so as not to be ejected during centrifugation. Causes the rotor to be unbalanced, embroidered or corroded. Covered sealed centrifuge tubes for preparative ultracentrifuges generally require that the liquid be filled to prevent deformation of the upper portion of the plastic centrifuge tube due to vacuum depression during centrifugation.
When the centrifuge angle rotor is loaded with the sample, it is necessary to balance the layout and strictly control the sample loading quality, and the sample must be evenly distributed. When using the flattened rotor body, in addition to the balanced loading of the sample, the basket and the fittings need to be trimmed first to ensure the overall balance of the rotor body system. Tubes and sample containers used for centrifugation should always be tightly capped (** screw caps); remove stains from the centrifuge sleeve, rotor and centrifuge chamber after each use. If specimen spillage occurs: First, spray a disinfectant containing an effective chlorine concentration of 5000mg/L to the specimen spilling place, disinfect for 30 minutes, then use a pair of forceps to absorb the mixture of contaminant and disinfectant and discard it. In the waste bin, ** clean the sample overflow area with a disinfectant containing an effective chlorine concentration of 5000 mg/L. The centrifuge can be used again after returning to dryness.
