The role of freezing in centrifugation during experiments.

Release time:

2011-12-27

Overview:

Biological activity is most vigorous at 4°C. To maintain the temperature inside the centrifuge chamber at 4°C, it is necessary to use a centrifuge equipped with a refrigeration function.

　　Some bioactivity assays require that the biological activity of the experimental substance be maintained. However, biological activity is most vigorous at 4°C. To keep the temperature inside the centrifuge chamber at 4°C, it is necessary to use a centrifuge equipped with a refrigeration function.
　　Generally, when selecting a centrifuge for bioactive cell experiments, there are many different types available; however, the two main categories are high-capacity refrigerated centrifuges and high-speed refrigerated centrifuges. When choosing a specific model, it’s usually necessary to select based on the particular requirements of the experiment.
　　The following lists the basic configuration requirements for a high-speed refrigerated centrifuge needed for DNA extraction:
　　Maximum speed: 16,000 rpm
　　Maximum relative centrifugal force: 17,800 × g
　　No. 1 conical rotor, 1.5/2.2 ml × 12 (16,000 r/min), RCF: 17,800 × g
　　No. 2 conical rotor, 1.5/2.2 ml × 24 (13,200 rpm), RCF: 16,110 × g
　　4-Position Horizontal Enzyme-Linked Immunosorbent Assay Rotator, 2×48 Wells (3000 rpm), RCF: 1400×g
　　No. 5 conical rotor, 10 ml × 12 (12,000 rpm), RCF: 14,300 × g
　　Speed accuracy: ±50 r/min
　　Temperature range: -20℃ to +40℃
　　Temperature control accuracy: ±1.5℃
　　Compressor: High-performance compressor unit with imported components, using non-fluorinated refrigerant R404a.
　　Timing range: 0–99 minutes
　　The above information is provided by Shanghai Luxiangyi Centrifuge. Please cite the source website when reprinting.