The cell centrifuge separates the cells by centrifugation, which is a substance separation. Without passing through the medium, there is no secondary pollution.
The cell centrifuge uses the centrifugal separation purification method:
Centrifugation methods, including differential centrifugation and density gradient centrifugation, are further divided into velocity-zone density gradient centrifugation and iso-density gradient centrifugation. After selecting a reasonable method to break the cells or tissues, the differential centrifugation method is first performed, that is, the various cell components and particles are separated according to different centrifugal forces generated by different centrifugal speeds. Typical differential centrifugation sequences for various organelles and particles are shown in the table below.
A cell centrifuge is a special instrument that separates and precipitates a mixture (containing solids) by centrifugal force. Cell centrifuges are available in low speed, high speed centrifuges and low speed, cell centrifuges, as well as overspeed analysis and dual-purpose refrigerated centrifuges. The cell centrifuge is a high speed centrifuge with a refrigeration system. The centrifuge chamber temperature of such centrifuges typically drops below zero degrees Celsius. The rotational speed can reach 10,000 rpm or more. Besides the performance and structure of the refrigerated centrifuge, the angle rotor used in the cell centrifuge is mostly made of titanium alloy or aluminum alloy. The centrifuge tube is a polyethylene hard plastic product with a cover. Such centrifuges are often used to collect microorganisms, cell debris, cells, large organelles, sulfate precipitates, and immunoprecipitates. So, what are the issues that need to be focused on when operating a cell centrifuge?
Things to watch out for when operating a cell centrifuge:
1. The amount of solution loaded in the centrifuge tube must be appropriate. The stainless steel tube has no cover and can only be installed 2/3. The plastic tube can be attached to the shoulder. The cover must be covered and not allowed to leak. Empty tube centrifugation will deform. The use of organic solvents in plastic pipes must comply with the regulations.
2. Centrifuge tubes must be placed in pairs and must be strictly balanced with a deviation of <0.1g.
3. No idling is allowed, and the handle must be used to prevent the rotor from slipping. Turn the head to be light and steady, and when you turn the handle down, hold the handle with your hand and turn the head only. The rotating head cover should be tightly closed, and the cover is not allowed to be centrifuged.
4. When the cell centrifuge is centrifuged, the cover is not allowed to open. It is not allowed to be supported on the centrifuge. If there is abnormal sound and vibration, stop immediately.
5. After using the rotor, it must be taken out from the rotor room in time, wiped dry, and carefully cleaned with the hole rod. If there is a solution overflow, it must be cleaned, wipe the condensate inside the rotor, open the door and dry the rotor room.
6. The centrifuge must be reserved and must be registered after use.
7. Those who have not been trained and assessed cannot use it.
8. Choose the right rotor and speed, never use it.
9. Choose the right temperature, usually 4 ° C, do not lower than the organic solvent, so as not to freeze, damage the centrifuge tube and rotor.
